Laboratory Exercises in Microbiology – Lansing M. Prescott, John P. Harley – 5th Edition

1 Microscopic Techniques

1 Bright-Field Light Microscope and Microscopic Measurement of Organisms

2 The Hanging Drop Slide and Bacterial Motility

3 Dark-Field Light Microscope

4 Phase-Contrast Light Microscope

5 Flourescence Microscope

2 Bacterial Morphology and Staining

6 Negative Staining

7 Smear Preparation and Simple Staining

8 Gram Stain

9 Acid-Fast Staining (Ziehl-Neelsen and Kinyoun) Procedures

10 Endospore Staining (Schaeffer-Fulton or Wirtz-Conklin)

11 Capsule Staining

12 Flagella Staining: West and Difco's SpotTest Methods

3 Basic Laboratory and Culture Techniques

13 Microbiological Culture Media Preparation and Sterilization

14 Culture Transfer Instruments, Techniques, and Isolation and Maintenance of Pure Cultures

15 Spread-Plate Technique

16 The Streak-Plate Technique and Different Media

17 Pour-Plate Technique

18 Cultivation of Anaerobic Bacteria

19 Determination of Bacterial Numbers

4 Biochemical Activities of Bacteria

20 Carbohydrates I: Fermentation and B-Galactosidase Activity

21 Carbohydrates II: Triple Sugar Iron Agar Tests

22 Carbohydrates III: Starch Hydrolysis

23 Lipids: Lipid Hydrolysis

24 Proteins, Amino Acids, and Enzymes I: Hydrogen Sulfide Production and Motility

25 Proteins, Amino Acids, and Enzymes II: The IMViC Tests

26 Proteins, Amino Acids, and Enzymes III: Casein Hydrolysis

27 Proteins, Amino Acids, and Enzymes IV: Gelatin Hydrolysis

28 Proteins, Amino Acids, and Enzymes V: Catalase Activity

29 Proteins, Amino Acids, and Enzymes VI: Coagulase and DNase Activity

30 Proteins, Amino Acids, and Enzymes VII:Oxidase Test

31 Proteins, Amino Acids, and Enzymes VIII: Urease Activity

32 Proteins, Amino Acids, and Enzymes IX: Lysine and Ornithine Decarboxylase Test

33 Proteins, Amino Acids, and Enzymes X: Phenylalanine Deamination

34 Proteins, Amino Acids, and Enzymes XI: Nitrate Reduction

5 Rapid Multitest Systems

35 The API 20E System

36 The Enterotube II System

6 Unknown Identification

37 Using the First Edition of Bergey's Manual of Systematic Bacteriology to Identify Bacteria

38 General Unknown

7 Environmental Factors Affecting Growth of Microorganisms

39 Temperature

40 pH

41 Osmotic Pressure

42 The Effects of Chemical Agents on Bacteria I: Disinfectants

43 The Effects of Chemical Agents on Bacteria II: Antimicrobial Agents (Kirby-Bauer Method)

44 Hand Washing, Environmental Sampling, and Microbiological Monitoring

45 Determination of a Bacterial Growth Curve: Classical and Two-Hour Methods

8 Environmental and Food Microbiology

46 Standard Coliform Most Probably Number (MPN)Test and Presence-Absence Coliform Test

47 Membrane Filter Technique for Coliform and Fecal Streptococci; KONFIRM Test for Fecal Coliforms

48 Isolation of Escherichia coli Bacteriophages from Sewage and Determining Bacteriophage Titers

49 Enumeration of Soil Microorganisms

50 Bacterial Count of a Food Product

51 Examination of Milk for Bacteria

9 Medical Microbiology

52 Agglutination Reactions: Blood Groups

53 Isolation of Normal Microbiota from the Human Body

54 Staphylococci

55 Pneumococci

56 Streptococci

57 Neisseriae

58 Aerobic and Anaerobic Endospore-Forming Bacteria

10 Survey of Selected Eucaryotic Microorganisms

59 Fungi I: Yeasts

60 Fungi II: Phycomycetes, Ascomycetes, and Basidiomycetes

11 Microbial Genetics and Genomics

61 Bacterial Mutation

62 Bacterial Transformation

63 Bacterial Conjugation: The Transfer of Antibiotic-Resistant Plasmids

64 Isolation of Genomic DNA from Saccharomyces cerevisiae

65 Isolation and Purification of Genomic DNA from Escherichia coli

66 Identifying Archaea and Bacteria Using the Internet and Computer-Assisted Gene Analysis

Appendix A Dilutions with Sample Problems

Appendix B Metric and English Measurement Equivalents

Appendix C Transmission-Absorbance Table for Spectrophotometry

Appendix D Logarithms

Appendix E pH and pH Indicators

Appendix F Scientific Notation

Appendix G Identification Charts

Appendix H Reagents, Solutions, Stains, and Tests

Appendix I Culture Media

Appendix J Sources and Maintenance of Microbiological Stock Cultures